Genotyping of human bocavirus using a restriction length polymorphism
Identifieur interne : 003997 ( Main/Exploration ); précédent : 003996; suivant : 003998Genotyping of human bocavirus using a restriction length polymorphism
Auteurs : Vanessa Ditt [Allemagne] ; Sergei Viazov [Allemagne] ; Ramona Tillmann [Allemagne] ; Verena Schildgen [Allemagne] ; Oliver Schildgen [Allemagne]Source :
- Virus Genes [ 0920-8569 ] ; 2008-02-01.
English descriptors
- KwdEn :
Abstract
Abstract: Sequencing analysis of the isolates of a recently identified pathogen associated with respiratory infections, human bocavirus (HBoV), allowed for identification of two virus genotypes of the virus. In the current article a new method for a simple and fast differentiation of HBoV genotypes in clinical materials is described. The test includes an amplification of a 309 bp fragment of VP1/VP2 gene of HBoV from nasopharyngeal aspirates with a subsequent incubation of a PCR mix with the BstAPI endonuclease. Upon such a digestion, the DNA fragment derived from the genotype I HBoV isolates forms two fragments of 150 and 159 bp, while that obtained from genotype 2 isolates remains unrestricted. The developed technique may be used in epidemiological studies of HBoV infection and analysis of the potential differences in biological characteristics of HboV genotypes.
Url:
DOI: 10.1007/s11262-007-0182-0
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000F18
- to stream Istex, to step Curation: 000F18
- to stream Istex, to step Checkpoint: 001341
- to stream Main, to step Merge: 003B52
- to stream Main, to step Curation: 003997
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genotyping of human bocavirus using a restriction length polymorphism</title><author><name sortKey="Ditt, Vanessa" sort="Ditt, Vanessa" uniqKey="Ditt V" first="Vanessa" last="Ditt">Vanessa Ditt</name></author><author><name sortKey="Viazov, Sergei" sort="Viazov, Sergei" uniqKey="Viazov S" first="Sergei" last="Viazov">Sergei Viazov</name></author><author><name sortKey="Tillmann, Ramona" sort="Tillmann, Ramona" uniqKey="Tillmann R" first="Ramona" last="Tillmann">Ramona Tillmann</name></author><author><name sortKey="Schildgen, Verena" sort="Schildgen, Verena" uniqKey="Schildgen V" first="Verena" last="Schildgen">Verena Schildgen</name></author><author><name sortKey="Schildgen, Oliver" sort="Schildgen, Oliver" uniqKey="Schildgen O" first="Oliver" last="Schildgen">Oliver Schildgen</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D6B266600A0E75A03ED26B75536C9DA6E8BE04F4</idno><date when="2007" year="2007">2007</date><idno type="doi">10.1007/s11262-007-0182-0</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-32LSDTB3-7/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000F18</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F18</idno><idno type="wicri:Area/Istex/Curation">000F18</idno><idno type="wicri:Area/Istex/Checkpoint">001341</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001341</idno><idno type="wicri:doubleKey">0920-8569:2007:Ditt V:genotyping:of:human</idno><idno type="wicri:Area/Main/Merge">003B52</idno><idno type="wicri:Area/Main/Curation">003997</idno><idno type="wicri:Area/Main/Exploration">003997</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Genotyping of human bocavirus using a restriction length polymorphism</title><author><name sortKey="Ditt, Vanessa" sort="Ditt, Vanessa" uniqKey="Ditt V" first="Vanessa" last="Ditt">Vanessa Ditt</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Virology, University of Bonn, Sigmund-Freud-Strasse 25, 53105, Bonn</wicri:regionArea><placeName><settlement type="city">Bonn</settlement><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region></placeName></affiliation></author><author><name sortKey="Viazov, Sergei" sort="Viazov, Sergei" uniqKey="Viazov S" first="Sergei" last="Viazov">Sergei Viazov</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Virology, University Hospital Essen, Essen</wicri:regionArea><wicri:noRegion>Essen</wicri:noRegion><wicri:noRegion>Essen</wicri:noRegion></affiliation></author><author><name sortKey="Tillmann, Ramona" sort="Tillmann, Ramona" uniqKey="Tillmann R" first="Ramona" last="Tillmann">Ramona Tillmann</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Virology, University of Bonn, Sigmund-Freud-Strasse 25, 53105, Bonn</wicri:regionArea><placeName><settlement type="city">Bonn</settlement><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region></placeName></affiliation></author><author><name sortKey="Schildgen, Verena" sort="Schildgen, Verena" uniqKey="Schildgen V" first="Verena" last="Schildgen">Verena Schildgen</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Virology, University of Bonn, Sigmund-Freud-Strasse 25, 53105, Bonn</wicri:regionArea><placeName><settlement type="city">Bonn</settlement><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region></placeName></affiliation></author><author><name sortKey="Schildgen, Oliver" sort="Schildgen, Oliver" uniqKey="Schildgen O" first="Oliver" last="Schildgen">Oliver Schildgen</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Virology, University of Bonn, Sigmund-Freud-Strasse 25, 53105, Bonn</wicri:regionArea><placeName><settlement type="city">Bonn</settlement><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2008-02-01">2008-02-01</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="67">67</biblScope><biblScope unit="page" to="69">69</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bocavirus</term><term>Genotyping</term><term>Respiratory infection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Sequencing analysis of the isolates of a recently identified pathogen associated with respiratory infections, human bocavirus (HBoV), allowed for identification of two virus genotypes of the virus. In the current article a new method for a simple and fast differentiation of HBoV genotypes in clinical materials is described. The test includes an amplification of a 309 bp fragment of VP1/VP2 gene of HBoV from nasopharyngeal aspirates with a subsequent incubation of a PCR mix with the BstAPI endonuclease. Upon such a digestion, the DNA fragment derived from the genotype I HBoV isolates forms two fragments of 150 and 159 bp, while that obtained from genotype 2 isolates remains unrestricted. The developed technique may be used in epidemiological studies of HBoV infection and analysis of the potential differences in biological characteristics of HboV genotypes.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Cologne</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Bonn</li></settlement></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Ditt, Vanessa" sort="Ditt, Vanessa" uniqKey="Ditt V" first="Vanessa" last="Ditt">Vanessa Ditt</name></region><name sortKey="Schildgen, Oliver" sort="Schildgen, Oliver" uniqKey="Schildgen O" first="Oliver" last="Schildgen">Oliver Schildgen</name><name sortKey="Schildgen, Oliver" sort="Schildgen, Oliver" uniqKey="Schildgen O" first="Oliver" last="Schildgen">Oliver Schildgen</name><name sortKey="Schildgen, Verena" sort="Schildgen, Verena" uniqKey="Schildgen V" first="Verena" last="Schildgen">Verena Schildgen</name><name sortKey="Tillmann, Ramona" sort="Tillmann, Ramona" uniqKey="Tillmann R" first="Ramona" last="Tillmann">Ramona Tillmann</name><name sortKey="Viazov, Sergei" sort="Viazov, Sergei" uniqKey="Viazov S" first="Sergei" last="Viazov">Sergei Viazov</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003997 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003997 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:D6B266600A0E75A03ED26B75536C9DA6E8BE04F4
|texte= Genotyping of human bocavirus using a restriction length polymorphism
}}
| This area was generated with Dilib version V0.6.33. |  |